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  • CRISPR-miRNA Synergy: Editing Tumor Suppressor miRNAs into Oncogenic "Dead Zones"

  • Mahdi Ferdosipour,1,*
    1. Islamic Azad University, Tehran Medical Sciences branch


  • Introduction: Tumor suppressor miRNAs are frequently silenced in cancer, contributing to therapy resistance. CRISPR/Cas9 offers a novel approach to reactivate these miRNAs by targeting oncogenic "dead zones" - resistant cancer regions inaccessible to conventional treatments.
  • Methods: We reviewed studies (2010-2024) using CRISPR/Cas9 to edit tumor suppressor miRNAs (miR-34a, miR-145, miR-200) in aggressive cancers. Analysis included delivery systems, editing efficiency, and functional outcomes from in vitro and xenograft models.
  • Results: CRISPR-mediated miRNA restoration achieved: 3-5-fold expression increase - 65-89% tumor volume reduction - 82% increased apoptosis - Effective targeting of resistant oncogenic hubs Challenges included off-target effects (5-15%) addressed by improved delivery systems.
  • Conclusion: CRISPR-miRNA synergy successfully targets oncogenic dead zones, offering a promising precision oncology approach. Future work should optimize delivery and validate clinical applications.
  • Keywords: CRISPR-Cas9, Tumor suppressor miRNAs, Oncogenic dead zones, Precision oncology

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