• info@icbcongress.com
• English
• Persian

• Login

Create Free Account

INTERNATIONAL CONGRESS OF

CancerGenetics

• Systems biology research and bioinformatic analysis of PPP3R1 RNA interaction and expression analysis in non-small cell lung cancer (NSCLC)

• Fatemeh Salehi,¹ Ali Ghaneh,^2,*
  1. Department of Sciences and Biotechnology, Shahid Ashrafi Isfahani University, Isfahan,Iran
  2. Department of Sciences and Biotechnology, Shahid Ashrafi Isfahani University, Isfahan,Iran
  
  
  
• Introduction: Lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) are the two most common types of lung cancer. In the past, they were grouped together under the name non-small cell lung cancer (NSCLC) and treated the same way. However, new studies suggest that LUAD and LUSC are different diseases and should be treated separately. Still, the specific clinical differences between LUAD and LUSC have not been fully explained yet (1)(2). The mitoribosome's regulatory subunit MRPL35 can control how cytochrome c oxidases assemble and is crucial for the development of non-small cell lung cancer (NSCLC). Knockdown of MRPL35 suppressed cell proliferation and decreased NSCLC progression both in vitro and in vivo. The possible molecular mechanisms were also clarified, which indicated that MRPL35 could be involved in cell apoptosis and proliferation by modulating the expression levels of CDK1, BIRC5, CHEK1, STMN1 and MCM2
• Methods: Microarray analysis (GSE225959) was used to identify the key protein-coding gene affected in NSCLC. Validation of these findings and survival analysis were conducted using ENCORI. RNA and protein interactions were explored through miRWalk, lncRRIsearch, and STRING. The Enrichr and Reactome databases were employed to identify relevant signaling pathways.(4)
• Results: Based on microarray analysis, PPP3R1 is significantly overexpressed in NSCLC. PPP3R1 modulates signaling pathways such as Signal Transduction and PI3K/AKT Signaling in cancer. lncRNAs NEAT1 and AATBC interact with PPP3R1 mRNA. Additionally, miR-22-3p suppresses the expression of PPP3R1 by binding to its 3’ UTR region.
• Conclusion: miR-22-3p, along with lncRNAs NEAT1 and AATBC, regulate Signal Transduction and PI3K/AKT signaling in cancer through their interaction with PPP3R1. PPP3R1, NEAT1, and AATBC may be considered potential diagnostic biomarkers for non-small cell lung cancer (NSCLC).
• Keywords: Microarray Analysis Diffraction expression analysis, non small cell lung cancer