INTERNATIONAL CONGRESS OF
CancerGenetics
Investigation of the relationship between circ_0000745 (SPECC1) expression and has-miR-145-5p in tissue samples of patients with breast adenocarcinoma
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Farshid Ardabili,1 Negisa Rahmani,2 Mahsa Azimi,3 Zohre Jahanafrooz,4,* Sahar Moghbelinejad,5
1. Department of Cell and Molecular Biology, University of Maragheh
3. Department of Medical laboratory sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
4. Department of Biology, Faculty of Sciences, University of Maragheh, Maragheh, Iran
5. Cellular and Molecular Research Centre, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
- Introduction: Breast cancer is one of the most common cancers among women, and early diagnosis plays a key role in improving the prognosis of the disease. In recent years, non-coding RNAs, including circRNAs and miRNAs, have emerged as potential biomarkers for cancer diagnosis and prognosis. circ_0000745 (SPECC1) and hsa-miR-145-5p are two important molecules involved in the regulation of genes related to breast cancer, which may play critical roles in cell proliferation and cancer progression.
- Methods: In this study, tumor tissue and normal tissue samples were collected from patients with breast adenocarcinoma. Total RNA was extracted from tissue samples, and cDNA synthesis was subsequently performed. The expression levels of circ_0000745 and hsa-miR-145-5p were then assessed using qRT-PCR. Additionally, the relationship between the expression of these RNAs and the clinical characteristics of the patients was examined.
- Results: The findings revealed a significant upregulation of circ_0000745 in cancerous tissues compared to normal tissues. In contrast, hsa-miR-145-5p was significantly downregulated in tumor tissues. These results suggest a potential role for these two molecules in regulating processes related to cell proliferation and migration in breast cancer.
- Conclusion: The upregulation of circ_0000745 and downregulation of hsa-miR-145-5p may serve as a novel biomarker pattern for the diagnosis and prognosis of breast cancer. These findings indicate that the circRNA-miRNA axis could be a potential therapeutic target in breast cancer treatment, warranting further investigation.
- Keywords: Breast cancer Circular RNA MicroRNA Gene expression Non-coding RNA
