INTERNATIONAL CONGRESS OF
CancerGenetics
Investigating different stages of esophageal cancer differentiation using histochemical technique
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Mohammadreza Pourmohammad,1 Khadijeh Afshoun,2 Jina Khayatzadeh,3,* Sepideh Salari,4 Alireza Khoei,5 Alireza Fazel,6
1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
2. Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
5. Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
- Introduction: Esophageal Squamous Cell Carcinoma (ESCC) is the seventh most common and lethal malignancy worldwide with a high incidence in Iran. Esophageal cancer has a poor prognosis and low 5-years survival rate. Despite several studies on esophageal cancer, its underlying mechanism remains unclear. This study was conducted in order to investigate histochemistry and its relationship with tumor differentiation in esophageal carcinoma.
- Methods: Tissue samples of esophageal carcinoma of 40 patients with different differentiations (well, moderate, poorly and undifferentiated) along with healthy esophageal samples were selected from the pathology department of Imam Reza Hospital (AS) in Mashhad after studying hematoxylin and eosin slides. To ensure the previous diagnosis, 5 micrometer thick sections were prepared and stained with Alcin Blue with PH 1 and 2.5 (to identify sulfated and carboxylated acid glycoproteins) and examined microscopically. The results were analyzed and evaluated by color intensity table (according to Gong method) and Kruskal-Wallis statistical analysis.
- Results: The results showed that sulfated and carboxylated acidic mucous compounds are not present in any of the different stages of esophageal squamous cell carcinoma differentiation, similar to normal tissue, while the reaction of tumor stroma and esophageal mucous glands was positive for the above compounds.
- Conclusion: It seems that in the cancerous process, changes in the function of esophageal lining cells that lead to changes in the production of sulfated and carboxylated mucous compounds do not occur.
- Keywords: esophageal carcinoma, histochemistry, cell differentiation, Alcin blue
