INTERNATIONAL CONGRESS OF
CancerGenetics
Using histochemistry technique to detect various differentiation stages of gastric carcinoma
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Mohammadreza Pourmohammad,1 Khadijeh Afshoun,2 Jina Khayatzadeh,3,* Mino gohari,4 Alireza Khoei,5 Alireza Fazel,6
1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
2. Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
5. Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
- Introduction: Gastric carcinoma is one of the most abundant and malignant cancers, and early diagnosis of the disease is very effective in the treatment method and survival rate. This study was conducted in order to investigate histochemistry and its relationship with tumor differentiation in gastric carcinoma.
- Methods: Tissue samples of gastric carcinoma of 40 patients with different differentiations (well, moderate, poorly and undifferentiated) along with healthy gastric samples were selected from the pathology department of Imam Reza Hospital (AS) in Mashhad after studying hematoxylin and eosin slides. To ensure the previous diagnosis, 5 micrometer thick sections were prepared and stained with Alcin Blue with PH 1 and 2.5 (for sulfated and carboxylated mucous acid compounds) and examined microscopically. The results were analyzed and evaluated by color intensity table (according to Gong method) and Kruskal-Wallis statistical analysis.
- Results: The results of Alcin blue with pH 1 staining showed a positive response in the stroma of normal gastric mucosa and tumor stroma, while gastric gland cells and tumor cells in different differentiations showed a negative response to this pH. In Alcin Blue 2.5 staining, the response of healthy glandular cells was intense, which decreased with the decrease of tumor differentiation and the increase of malignancy.
- Conclusion: Probably, the genetic changes in gastric cancer cells have led to the reduction of carboxylated mucous compounds compared to the healthy sample, but in normal and cancerous cells of the stomach, apparently, the lack of synthesis of sulfated mucous compounds can be suggested.
- Keywords: Gastric carcinoma, histochemistry, cell differentiation, Alcin blue
