INTERNATIONAL CONGRESS OF
CancerGenetics
Evaluating combinatorial effects of stylosin and radiotherapy on human glioblastoma cells
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Mohamad Vosough Ghanbari,1 Hamid Gholamhosseinian,2 Fatemeh Behnam Rassouli,3,* Khadijeh Jamialahmadi,4
1. Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2. Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3. Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
4. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
- Introduction: Stylosin, a monoterpene compound extracted from Ferula species, exhibits valuable pharmaceutical effects, such as anticancer properties. Glioblastoma is the most aggressive and common type of malignant brain tumor that originates from glial cells. Despite the routine use of surgery, chemotherapy and radiotherapy for treating glioblastoma, the survival rate remains low.
- Methods: The aim of present study was to study toxic effects of stylosin and radiotherapy on human glioblastoma cells for the first time. In this regard, U87 cells were treated with increasing concentrations of stylosin (20, 40, 60, 80 and 100 µM) during five consecutive days and viability was evaluated by alamarBlue assay. Upon determining the optimum concentrations of stylosin, cells were treated with 60 and 80 µM stylosin for 48 h, then exposed to 4, 6 and 8 Gy ionizing radiation (IR) and recovered for 72 h. To note, cells only exposed to IR and recovered for 72 h were also considered as relevant controls.
- Results: Findings revealed 84% and 42% viability for U87 cells upon 120 h treatment with 60 and 80 µM stylosin, respectively. Cotreatment of glioblastoma cells with 60 µM stylosin and 8 Gy IR decreased viability down to 76%. Intriguingly, effects of 80 µM stylosin were more considerable, as viability of cells was reduced to 10%, 11% and 10% after irradiation at 4, 6 and 8 Gy, respectively.
- Conclusion: Current findings suggest that stylosin could be considered as a potent agent for combinatorial treatment of glioblastoma.
- Keywords: Stylosin, Radiotherapy, Glioblastoma, Combination.
