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INTERNATIONAL CONGRESS OF

CancerGenetics

• effect of pomegranate extract on mcf-7 and lymphocyte

• nafiseh paysokhan,^1,* arefeh majidi khamene,² zahra Matluobi,³
  1. Student Research Committee, Sabzevar university of Medical Sciences, Sabzevar, Iran
  2. Student Research Committee, Sabzevar university of Medical Sciences, Sabzevar, Iran
  3. Cellular and Molecular Research Center, Sabzevar university of Medical Sciences, Sabzevar, Iran
  
  
  
• Introduction: Breast cancer is one of the problems of today's world, which has caused many deaths, and these deaths often occur due to the drug resistance of cancer cells to common breast cancer treatments. The use of an efficient auxiliary drug along with common cancer treatments can break this drug resistance and reduce its effects. One of the accessible and harmless suggestions are herbal medicines. Iran can improve in this field due to its favorable climate. Pomegranate, which is a fruit with antioxidant properties, is one of the suggestions. This fruit seems to be effective in the more effective treatment of cancer cells due to the presence of allagitanin, which has apoptotic properties. In these studies, we investigated the effects of pomegranate extract on breast cancer in mcf-7 cell line and also its effects on lymphocyte cells.
• Methods: The MCF7 cells were cultured in DMEM, supplemented with 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin. Cells were maintained in a humidified incubator at 37°C with 5% CO2. Then the cells were treated with different concentrations and mtt test was performed for them. The best drug concentrations were extracted using the kit, their RNA was extracted, and then cDNA was made, and real time was performed by designed primers. Lymphocyte cells, after being extracted from fresh blood, were poured into the plate using Ficoll and then treated with specific concentrations. After 24 hours, mtt test was performed and the best concentrations were analyzed after rna extraction and cDNA production by real time and designed primers. In the end, all the results obtained from mcf7 cells and lymphocytes were examined and analyzed using Linerg and Genex software.
• Results: The real time results of mcf-7 cells showed that the ratio of bax/bcl2 has increased significantly, which indicates the good effect of this extract. Also, the mtt results of lymphocyte cells showed that these cells multiplied when exposed to pomegranate extract, as well as the expression level bcl2 was increased compared to bax in these cells.
• Conclusion: Pomegranate extract seems to have a good effect on this type of cancer cell and can act as a treatment aid along with other treatments. Also, this extract has a good effect on lymphocyte cells and acts as a lymphocyte cell booster. Further research on other cancer cell lines needs to be investigated.
• Keywords: breast cancer/ pomegranate extract/ mcf7/ bax/ bcl2