INTERNATIONAL CONGRESS OF
CancerGenetics
Cytotoxic effect of sesquiterpene lactone britannin on colorectal cancer cells in-vitro
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Maryam Hamzeloo-Moghadam,1,* Sadegh Rajabi,2
1. Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1516745811, Iran
2. Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1434875451, Iran
- Introduction: Colorectal cancer (CRC) is the second leading cause of cancer deaths in the United States. Britannin, a sesquiterpene lactone, has demonstrated anticancer properties across various cancers. This study focused on assessing the antiproliferative effects of britannin on the HT29 colorectal cancer cell line.
- Methods: The antiproliferative effect of britannin on HT29 cells was assessed using the MTT method. HT29 cells were seeded in 96-well microplates and allowed to adhere for 24 hours. They were then treated with various concentrations of britannin (0-200 µM) for 24, 48, and 72 hours. After discarding the medium, the cells were incubated with MTT solution for 4 hours. The MTT solution was then replaced with a 1% DMSO solution. Finally, absorbance of the formazan crystals was measured at 570 and 630 nm to calculate the proliferation rate of HT29 cells.
- Results: Treatment of HT29 cells with various concentrations of britannin for 24 hours significantly reduced the viability percent, yielding an IC50 of 28.6 µM. After 48 hours, britannin treatment further inhibited cell viability, resulting in an IC50 of 20.9 µM compared to controls. Additionally, 72 hours post-treatment, the sesquiterpene lactone showed a notable decrease in HT29 cell viability with an IC50 of 21.3 µM.
- Conclusion: The MTT assay results indicated that britannin may exert a significant cytotoxic effect on human HT29 cells through its anti-proliferative activity, suggesting its potential as a natural compound for suppressing colorectal cancer growth.
- Keywords: Britannin, Inula aucheriana, Colorectal cancer, Antiproliferation.
