INTERNATIONAL CONGRESS OF
CancerGenetics
Determining high-risk genotypes 16 and 18 of human papillomavirus by multiplex real-time PCR method in suspected cervical cancer samples
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Maedeh Nasr Esfahani,1,* Dr. Zahra Zamanzadeh,2 Dr. Hamed Fakhim,3
- Introduction: Human papilloma virus types 16 and 18 can be mentioned among the main causes of cervical cancer, which is a disease with a high prevalence, especially in developing countries. This study was conducted with the aim of identifying high-risk types 16 and 18 infection in women suspected of having cervical cancer who had referred to medical centers
- Methods: After sample collection and DNA extraction, detection was done by Multiplex Real Time PCR molecular method.
- Results: The results showed that among the 100 samples suspected of cervical cancer, 52% were positive with genotype 16, 19% were positive with genotype 18, 2% were both genotypes 16 and 18 at the same time, and the rest were negative, of course, this negativity is because we only We examined the two mentioned genotypes
- Conclusion: In this study, it was shown that for accurate and specific diagnosis of HPV test and determination of its genotype, instead of using expensive commercial kits that are used in laboratories all over the country, you can use proper primer and probe and Multiplex Real Time PCR method. The use of the probe is as sensitive and accurate as the ready-made kits, but it is more economical and since the lower the cost, the more people are able to use this test for screening, so it is possible to detect the infection in the early stages and before The incidence of cervical cancer was treated.
- Keywords: Cervical Cancer, Screening, Human Papilloma Virus, Real-time Multiplex PCR
