• info@icbcongress.com
• English
• Persian

• Login

Create Free Account

INTERNATIONAL CONGRESS OF

CancerGenetics

• Investigating the expression changes of PI3k/Akt and miR-181a-5p in Jurket cell line after treatment with Cassiopeia andromeda toxin

• Elias Amraei,¹ Narges Obeydi,^2,* Mohammad Javad Mousavi,³ Sheyda Jafari,⁴ Taraneh Hoseinnezhad,⁵
  1. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  2. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  3. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  4. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  5. Department of Hematology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  
  
  
• Introduction: Nowadays, the use of drugs of natural resources is being considered. Cassiopea andromeda is a member of the family of jellyfish called upside-down jellyfish. The anticancer capabilities of Cassiopea andromeda venom have been displayed in several studies. On the other hand, miR-181a-5p acts as an oncomiR or tumor suppressor in various cancer types by affecting multiple hallmarks of tumors. It causes the activation and increase in the expression level of PI3k/Akt pathway genes. Recent studies have reported that PI3k/Akt signaling pathways are frequently altered in human cancer. This study investigated the effect of Cassiopeia andromeda venom on the expression of miR-181a-5p, PI3k, and Akt genes in the Jurkat leukemic T-cell line.
• Methods: Jurkat T-cells were exposed with IC50 concentrations of Cassiopeia andromeda venom next to peripheral blood lymphocyte cells (PBMC) as a control in RPMI1640 medium, RNA was extracted and cDNA synthesis was performed, then the relative expression level was measured by qPCR method.
• Results: The expression level of miR-181a-5p decreased in both Jurkat T-cells and PBMC, while Expression levels of PI3k and Akt differed between examined groups, the expression of PI3k, and Akt genes increased in PBMC but decreased in Jurkat T-cells.
• Conclusion: Our results showed that Cassiopeia andromeda toxin decreased the simultaneous expression of PI3k/Akt and miR-181a-5p genes in Jurkat T-cells, so inhibiting PI3k/Akt activity may be a valuable approach to treat cancer and it is possible that it can be used as a drug in cancer treatment.
• Keywords: Cassiopeia andromeda, miR-181a-5p, PI3k/Akt, Jurkat cell line