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INTERNATIONAL CONGRESS OF

CancerGenetics

• Investigating the effect of jujube (high TPC) and cedar honey (low TPC) on the expression of cyclin D1 gene in MCF-7 breast cancer cell line

• Paria Karimi,¹ Milad Bideh,^2,*
  1. Faculty of Basic Sciences,Sufiyan Islamic Azad University,East Azerbaijan,Iran
  2. * Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran *Corresponding author. Email: milad.bideh73@gmail.com
  
  
  
• Introduction: Breast cancer is one of the most common cancers in women. Honey is an essential natural ingredient used in traditional medicine to cure a number of disorders. It possesses several antioxidants, anti-inflammatory, antibacterial, anti-cancer, and immune system regulating effects. This work used two distinct types of honey, (honey with high TPC (Total Phenolic Content) (jujube honey) and honey with low TPC (cedar honey)) to evaluate cyclin D1 gene expression in MCF-7 cell line. Cyclin D1 gene is activated in the G1 phase and is involved in tumorigenesis. This study was carried out to investigate the effect of jujube (high TPC) and cedar honey (low TPC) on the expression of cyclin d1 in the MCF-7 breast cancer cell line.
• Methods: MCF-7 cell line was cultured at RPMI-1640 supplemented with 10% FBS. After the MTT was determined. 104 cells were seeded into the each well of 96-well plate in RPMI-1640 under 37 °C and 5% CO2. Cells were treated with different concentrations of honey (0, 3, 6.25, 8, 10, 12.5, 15, 20 and 25 %) for 24 hours. Finally, cell mortality was assessed using MTT colorimetric assay. Also, 105 cells were seeded into a 12-well plate and treated with honey (0, 3% and 5%) for 24 hours. Then, all samples have gathered for extraction of RNA and were reverse transcripted to cDNA; after that, analyzing gene expression using the SYBR Green method and Real Time-PCR thermocycler (ABI-USA). The 2-ΔΔct was entered into the SPSS software for one-way ANOVA analysis.
• Results: The results of the MTT assay showed that the IC50 of high-TPC honey was in lower concentration of low-TPC honey on MCF-7 cells (5% and 9%, respectively). Real-time PCR results showed a significant decrease in mRNA levels at all concentrations (3%, and 5%) of high TPC honey while there was no significant decrease in the expression of the cyclin D1 gene in Low TPC honey. Data distribution in this process was downward (concentration-dependent manner) and data distribution was normal (P-Value ≤ 0.05 was approved).
• Conclusion: Previous researches have shown that honey's cytotoxicity can be attributed to phenolic and flavonoid compounds, as well as their antioxidant capabilities. Honey can cause cytotoxicity by raising caspase 3, caspase 8, caspase 9, and the proapoptotic protein Bax while lowering the anti-apoptotic protein Bcl2. Previous studies have shown that honey reduces ER activity in an ERE-dependent pathway, it may also exert its effect through the SP1 and cAMP-PKA pathways. This claim requires more empirical studies.
• Keywords: Honey,cyclin d1 ,breast cancer