INTERNATIONAL CONGRESS OF
CancerGenetics
Examining the expression of Circ_0000745 and its relationship with hsa-miR-335-5p in the pathogenesis of breast cancer through the study of tissue samples from women in hospitals in Qazvin province.
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Negisa Rahmani,1 Farshid Ardabili,2 Sahar Moghbelinejad,3,*
1. Department of Biology, School of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3. Cellular and Molecular Research Centre, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
- Introduction: Breast cancer is one of the most common cancers among women, and early diagnosis of this disease plays an essential role in improving its prognosis. In recent years, non-coding RNAs such as circRNAs and miRNAs have been recognized as possible biomarkers for cancer diagnosis and prognosis. Circ_0000745 (SPECC1) and hsa-miR-335-5p are two key molecules involved in the regulation of genes related to breast cancer and may be involved in cell proliferation and cancer progression.
- Methods: In this study, tumor and normal tissue samples were collected from patients with breast adenocarcinoma. After extracting total RNA from tissue samples, cDNA synthesis was performed. The expression level of circ_0000745 (SPECC1) and hsa-miR-335-5p was evaluated using qRT-PCR technique. Also, the relationship between the expression of these RNAs and the clinical characteristics of the patients was investigated.
- Results: The results showed that the expression of circ_0000745 (SPECC1) was significantly increased in cancer tissues, while hsa-miR-335-5p was significantly decreased in tumor tissues. These results indicate the possible role of these two molecules in regulating the processes of cell proliferation and migration in breast cancer.
- Conclusion: Increased expression of circ_0000745 (SPECC1) and decreased expression of hsa-miR-335-5p can be proposed as a new biomarker pattern for breast cancer diagnosis and prognosis. These findings suggest that the circRNA-miRNA axis may be a potential therapeutic target in the treatment of breast cancer and needs further research.
- Keywords: breast cancer Circular RNA microRNA Gene expression Non-coding RNA (ncRNA)
